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Showing posts from February, 2021

Estimation of total chlorophyll by Aron's method

Purpose:- Estimation of total chlorophyll by Arnon's method. Requirement:- Spectrophotometer Buchner's funnel, filter paper test tube, Fresh leaves. measuring cylinder pestle & mortar 80% Acetone  Formula:- 20.2 (A645) +802 A663) / 1000 x W Principle:- Chlorophyll is extracted in 80% acetone & the absorption at 660 nm& 645 nm are read in s spectrophotometer using the absorption co-efficient, the amount of chlorophyll is calculated Procedure:- 1) Weigh 1 gm of finely cut & well mixed representative sample of a leaf or fruit tissue into a clean mortar 2) Grind the tissue to a fine pulp with the addition of 20mil of 80% acetone   3) Centrifuge (5000 rpm for 5 min) & transfer the supernatant to a 100 ml volumetric flask  4) Repeat this procedure until the residue is colorless wash the mortar and pestle  thoroughly with 80% acetone and collect the clear washing in the volumetric flask 5) Make up the volume to 100 ml with 80% acetone  6) ...

Two staged RC coupled Amplifier

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Purpose: - (a) Study the amplifier combined with two RC components. (b) Set the curve of the frequency response. Therefore determines the bandwidth.   Requirements: - Transistor, function generator, resistors, capacitors, controlled power supply, multimeter, breadboard, connecting cables, etc.   Circuits Diagram:- Opinion: - The amplifier is the building block for many electrical systems. A single-stage amplifier is not enough to build an effective electronic system. When no. The amplifier class is used sequentially called multistage. Amplifier (or) amplifier with cascaded high gain can be obtained from multistage amplifying. Demonstrates how to pair a phase 2 amplifier using the RC-coupling system. This is the most widely used method. In this system, a signal built across the resistor RE (R2) of the first phase is connected below the second phase with a capacitor CE (C2). The coupling capacitor prevents the DC power of the first phase from reaching the base ...